Victorian Centre for Functional Genomics at Peter Mac

Overview

The VCFG is a high throughput screening facility that enables researchers Australia-wide the opportunity to become fully trained to perform screens across CRISPR, RNAi and Compound platforms. The lab has an extensive array of reagent libraries, automated liquid handling, plate reader and imaging instrumentation. Projects can be performed in 2D and 3D settings, with cell lines or patient derived materials. Every project is customised and analysed in house and managed using PRIME, our PRoject Information and Management Enterprise. 

Capabilities

Accessed from Compounds Australia Griffith University BYO compounds welcome

Cell embedding in 3D matrices Drug synergy and interaction studies Live imaging High content imaging Technology development

Single cell proteomics using the Isoplexis Isolight BYO compounds welcome

We are constantly developing new tools, identifying new reagents, methods to use them and ways to analyse and enrich datasets. This is done in house or in conjunction with collaborators. 2D cellular phenotyping, drug synergy interactions performed through high content imaging. 3D cell line, PDX or PDO automated embedding, growth characterisation, quantitation of structure size, viability and response to death or morphology inducing agents, drug screens and bright field and fluorescent imaging methodologies. Drug Seq in conjunction with the Molecular Genomics Core Facility at PeterMac, high throughput 96 well format, multiplexed transcriptional profiling (arrayed screening strategies, drugs, synthetic CRISPR, RNAi). Nucleofection, single guide CRISPR, or multiple targets together, high throughput liquid handling protocols to screen in 96 well format. Single cell proteomics using IsoPlexis 2D cellular phenotyping, drug synergy interactions performed through high content imaging. 3D cell line, PDX or PDO automated embedding, growth characterisation, quantitation of structure size, viability and response to death or morphology inducing agents, drug screens and bright field and fluorescent imaging methodologies. Drug Seq in conjunction with the Molecular Genomics Core Facility at PeterMac, high throughput 96 well format, multiplexed transcriptional profiling (arrayed screening strategies, drugs, synthetic CRISPR, RNAi). Nucleofection, single guide CRISPR, or multiple targets together, high throughput liquid handling protocols to screen in 96 well format. Single cell proteomics using IsoPlexis

Human whole genome arrayed sgRNA library (Horizon Discovery). CRISPR KO Brunello (human) and Brie (mouse) pooled libraries. CRISPRa Calabrese (human) and Caprano (mouse) pooled libraries. CRISPRi Docetto (human) and Dolomiti (mouse) pooled libraries. TransEDIT dual guide whole genome human arrayed viral library. Library amplification protocols and reagents. In-house NGS and analysis pipelines.

Fully customised cell phenotyping pipelines for screens in 2D and 3D

Equipment

Fully automated Cellomics CX7-LED and CX7-LZR, widefield, confocal, live-cell imaging up to 60x magnification Cytation C10 spinning disk confocal, Cytation 5, widefield imaging

Incucyte SX5 (red, green, orange channels) Cytation 5 via the BioSpa incubator

Cytation 5 and C10 both are sophisticated plate readers that have spectral monochromators, direct injection for kinetic measurements, luminescence, fluorescence and alpha screen enabled

Caliper ALH3000 and Janus G3 liquid handling robots for all transfection and speciality liquid handling applications Rastrum 3D bioprinter for embedding cells in hydrogels BioTek 406: three units that aspirate and dispense media, cells, fix and stain BioTek multi-flo: aspirate and dispense any plate type Tecan D300E digital drug dispenser

AMAXA 4D nucleofector and 96 well adaptor for high throughput Seahorse metabolic bioanalyser LiCONICs 220 stacking tower incubator EVOS Fl, bench top fluorescent microscope Barcode printer; all instruments are barcode-read enabled Dedicated and fully backed up server for data and image storage

Isoplexis Isolight - single cell proteomics

Access Information

The VCFG's primary mode of operation is 'researcher driven, staff assisted' where a researcher embeds within the facility and is trained on all aspects of their project. Fully serviced is possible but only where capacity exists. Some instruments require limited assistance once trained.

For further information visit www.petermac.org/research/core-facilities-and-services/victorian-centre-functional-genomics

Contacts

A/Prof Kaylene Simpson
Head, Victorian Centre for Functional Genomics
kaylene.simpson@petermac.org

Team Members

 Dr Susanne Ramm | Second-in-charge  | 3D organoid characterisation, screening, analysis, innovation

Jennii Luu  | Lab Manager | Automation specialist, 2D and 3D platforms

Dr Twishi Gulati | CRISPR Screening, iLAB management, Business Development

Dr Henry Beetham | Stem cell project, 2D projects, CRISPR screening

Karla Cowley | High content imaging and analysis, 2D platform

Dr Mark Li | 3D screen support, fee for service and analysis

Arthi Macpherson | Equipment training, specialist instruments, PRIME management

Robert Vary | Equipment training, screen support

Images

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