VCGS/MCRI Genomics Facility
- Victorian Clinical Genetics Service / Murdoch Children's Research Institute
- Transcriptomics • Epigenomics • Genomics • Single Cell
Overview
The VCGS/MCRI Genomics Facility offers a diverse portfolio of sequencing services on a clinical and research basis. We offer DNA and RNA extraction, Next Generation Sequencing assays (including sample QCs, library preparation, library QC, and sequencing; but currently excluding bioinformatics). The laboratory is NATA accredited and operates in accordance with NPAAC and ISO15189 standards.
Capabilities
Clinical Whole Genome Sequencing (human)
Whole Genome Sequencing (other organisms)
Clinical Whole Exome Sequencing (human)
Whole Exome Sequencing (other commercially available organisms)
Custom panels
Illumina GSA v3
Illumina GDA
Illumina GDACyto
Illumina EPIC
16S V4/V5, shotgun sequencing, WGS (bacterial genomes), metagenomics
CFTRbrary: highly sensitive, versatile, and low cost assay that allows the detection of many more CF variants, including the Poly-T and TG tract
Mitochondrial genome sequencing
Custom assays
Illumina compatible libraries only
Bulk RNAseq (total RNA, mRNA, low input, fragmented RNA, etc.)
10x Genomics single-cell RNA-seq
High throughput DNA extractions, RNA extractions (by agreement only)
ATACseq, ChIPseq, Illumina EPIC (methylation) array
ATACseq, ChIPseq, custom assays (by agreement only)
ClarioSTAR (2x), TECAN Infinite Nano+, SpectraMax M2
Illumina iScan
Intergra biosciences VIAflo 96, Hamilton Starlet, Hamilton NGS STAR, Agilent BRAVO
There are several options nucleic acid quantitation and quality control:
•Qubit 2.0 and 3.0 for genomic DNA quantification or emPCR enrichment check, as examples
•TapeStation 2200 and 4200, an automated alternative to traditional gel electrophoresis
•E-Gel agarose for electrophoresis
•QIAxcel
Equipment
Illumina MiSeq (x2), Illumina NextSeq500 (x1), Illumina Novaseq6000 (x2)
Applied Biosystems™ 3730XL DNA Analyzer (2x)
10x Genomics Chromium Controller
LightCycler® 480 Real-Time PCR System
Bio-Rad's Droplet Digital PCR (ddPCR) System
•Hamilton PCR STARlet assay ready workstation
•Hamilton NGS STAR for Library Prep assay ready workstation
•Agilent Bravo Automated Liquid Handling Platform
Access Information
For further information visit www.vcgs.org.au/tests
Contacts
General lab contact for extraction services
dna.extractions@vcgs.org.au
General lab contact for sequencing services
genomics@vcgs.edu.au
Jessica Riseley
Deputy Head of Nucleic Acid Extraction and Sequencing Technologies
Jessica.riseley@vcgs.org.au
Dr Stefanie Eggers
Head of Nucleic Acid Extraction and Sequencing Technologies
stefanie.eggers@vcgs.org.au
Team Members
Iwona Büttner | Team Leader of Nucleic acid Extraction and Sequencing Technologies - DNA Extraction | iwona.buettner@vcgs.org.au
Simone Rowley | Team Leader of Nucleic acid Extraction and Sequencing Technologies - Sequencing Platform | simone.rowley@vcgs.org.au
Justin Crockett | Team Leader of Nucleic acid Extraction and Sequencing Technologies - Sequencing Platform | justin.crockett@vcgs.org.au
Sandra Nicoletti | Project Officer | Sandra.nicoletti@vcgs.org.au
Matthew McLeod | Automation and Integration Specialist | matthew.mcleod@ vcgs.org.au
Simone Rowley | Team Leader of Nucleic acid Extraction and Sequencing Technologies - Sequencing Platform | simone.rowley@vcgs.org.au
Justin Crockett | Team Leader of Nucleic acid Extraction and Sequencing Technologies - Sequencing Platform | justin.crockett@vcgs.org.au
Sandra Nicoletti | Project Officer | Sandra.nicoletti@vcgs.org.au
Matthew McLeod | Automation and Integration Specialist | matthew.mcleod@ vcgs.org.au