Doherty Applied Microbial Genomics
- The University of Melbourne
- Functional Genomics • Transcriptomics • Microbiomics • Genomics
Overview
Within the framework of collaborative partnerships, Doherty Applied Microbial Genomics (DAMG) offers sequencing capacity, established expertise in bioinformatics and critical training to undertake microbial genomics research, which will help laboratories across Australia to breach barriers that are restricting access to this new cutting edge technology. The initiative is funded by the Department of Microbiology and Immunology at The University of Melbourne, and has a close alignment with the Microbiological Diagnostic Unit Public Health Laboratory (MDU PHL).
We have established expertise in bioinformatics and capacity to perform microbial genomics research make us uniquely placed to perform and facilitate research in this rapidly evolving area of clinical and public health microbiology.
Primary research themes include:
We have established expertise in bioinformatics and capacity to perform microbial genomics research make us uniquely placed to perform and facilitate research in this rapidly evolving area of clinical and public health microbiology.
Primary research themes include:
- Antimicrobial resistance,
- Infectious disease surveillance and one health, and
- Metagenomics and Software development.
Method specialties include:
- Amplicon 16S microbiota profiling,
- Shotgun metagenome sequencing, and
- Whole Genome Sequencing (WGS) on purified isolates.
Capabilities
Bacterial Whole Genome Sequencing (WGS)
Viral WGS
Metagenomics
Targeted WGS
High throughput bacterial DNA/RNA extraction
16S rRNA V4
16S rRNA full gene
Custom amplicons
Illumina
Oxford Nanopore Technology
Custom libraries
Oxford Nanopore Tecnnologies
PacBio
Bacterial
Metatranscriptomics
Clinical, Public health and Research Microbiology focused
Equipment
iSeq 100 Sequencing System
MiSeq System
NextSeq500/500
NextSeq 2000
Oxford Nanopore Technologies GridlON for on-demand sequencing with integrated real-time data processing
SMRT Cell Sequencing (Sequel I)
Perkin Elmer Zephyr for NGS applications
QIAsymphony
FLUOstar® Omega - multi-mode microplate reader with six detection modes, utilizing an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes.
Access Information
Research collaborations between your organisation and DAMG include project and experimental planning, all laboratory work and bioinformatics analysis and manuscript preparation.
For further information contact us at DAMG-general@unimelb.edu.au or submit a completed project proposal form to begin discussions.
Website: www.doherty.edu.au/genomics
For further information contact us at DAMG-general@unimelb.edu.au or submit a completed project proposal form to begin discussions.
Website: www.doherty.edu.au/genomics
Contacts
General contact
DAMG-general@unimelb.edu.au
Team Members
Benjamin Howden | Medical Director | b.howden@unimelb.edu.au
Tim Stinear | Scientific Director | t.stinear@unimelb.edu.au
Torsten Seemann | Lead Bioinfomatician | t.seemann@uniemlb.edu.au
Louise Judd | Senior Laboratory Scientist | lmj@unimelb.edu.au
Calum Walsh | Senior Bioinformatician | calum.walsh@unimelb.edu.au
Nicole Isles | Laboratory Scientist | isles.n@unimelb.edu.au
Geradline Kong | Laboratory Scientist | geraldine.kong@unimelb.edu.au
Tim Stinear | Scientific Director | t.stinear@unimelb.edu.au
Torsten Seemann | Lead Bioinfomatician | t.seemann@uniemlb.edu.au
Louise Judd | Senior Laboratory Scientist | lmj@unimelb.edu.au
Calum Walsh | Senior Bioinformatician | calum.walsh@unimelb.edu.au
Nicole Isles | Laboratory Scientist | isles.n@unimelb.edu.au
Geradline Kong | Laboratory Scientist | geraldine.kong@unimelb.edu.au