Peter Mac Molecular Genomics Core
- Peter MacCallum Cancer Centre
- Genomics • Transcriptomics • Epigenomics • Single Cell • Spatial
Overview
The Molecular Genomics Core at Peter Mac processes samples, implements and develops new genomics protocols for cancer research and translational applications. We work predominantly with Next Generation Sequencing (NGS, Illumina compatible) but also with the nCounter technology (Nanostring) for gene expression of FFPE-derived samples. We operate a Chromium iX for single-cell sequencing and the Visium (10xGenomics) and GeoMx DSP (Nanostring) for spatial transcriptomics. We specialize in custom protocols and challenging cancer samples such as low input and FFPE-derived.
Capabilities
Whole-genome sequencing (WGS)
Whole exome sequencing (WES)
DNA targeted capture (off-the-shelf and custom panels)
Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-Seq)
Chromatin immunoprecipitation followed by sequencing (ChIP-seq)
Methylated DNA immunoprecipitation sequencing (MeDIP-Seq)
Methylation
CRISPR-Seq
Support in custom applications
NextSeq 500 only
3'RNA-seq
RNA-Seq (polyA and ribo-depletion)
RNAseq and whole exome sequencing (WES)
SLAM-Seq
PRO-Seq
High-throughput low-cost RNA-Seq (MAC-Seq)
FFPE-derived RNA
Support in custom applications
Equipment
Chromium iX (10xGenomics)
Illumina NextSeq
Illumina NovaSeq
GeoMx DSP (Nanostring)
Visium (10X Genomics)
Bravo BenchCel Workstation
EpMotion
nCounter Nanostring for gene expression compatible with FFPE-derived RNA
Access Information
For further information visit www.petermac.org/research/core-facilities-and-services/molecular-genomics
Contacts
Tim Semple
Research Officer
tim.semple@petermac.org
General contact
ResearchMolecularGenomics@petermac.org
Team Members
- Tim Semple, Research Officer | Single cell genomics and spatial transcriptomics specialist | tim.semple@petermac.org
- Amanda Lee | Lab management and DNA targeted capture specialist | amanda.lee@petermac.org
- David Yoannidis | Custom protocols, DNA and RNA-seq specialist | david.yoannidis@petermac.org
- Stuart Craig | RNA specialist, DNA targeted capture | stuart.cragi@petermac.org
- Hugo Saunders | Nanostring specialist, GeoMx DSP specialist | hugo.saunders@petermac.org
- Sara Alaei | Single cell genomics specialist | sara.alaei@petermac.org