St Vincent's Institute Technologies Platform
- St Vincent's Institute of Medical Research
- Metabolomics • Proteomics • Single Cell • Transcriptomics
Overview
SVI focus on a wide range of disease-oriented research:
- Cancer
- Type 1 diabetes
- Type 2 diabetes and obesity
- Heart disease
- Bone diseases, including osteoporosis
- Alzheimer's disease
Our in-house tools include:
- Flow Cytometry (analysers + sorters)
- 3 × LC-MS for intact protein, proteomics & metabolomics analysis
- 10x Genomics Chromium controller
Capabilities
Protein Identification
Qualitative & quantitative proteome analysis
Molecular weight analysis of purified protein samples
Untargeted metabolomics
Nucleotides & Co-enzyme A (CoA) derivatives analysis
Construction of long-read (PacBio)
Short-read (Illumina) and single cell RNA sequencing libraries by several labs, especially the Genomics & Immunology laboratory
10x Genomics Chromium controller
Quanlitative analysis by high-resolution mass spectrometry
Drug purity check
C18 fractionation on semi-preparative HPLC
Bioinformatics capabilities located in within the following research groups:
1. Bioinformatics & Cellular Genomics,
2. Genomics & Immunology, and
3. Cancer & RNA Biology.
Equipment
10x Genomics Chromium controller
Numerous qPCR instruments, including ABI Quantstudio, Lightcycler, etc
Thermo Ultimate 3000 LC coupled with Orbitrap Fusion Lumos
Agilent 1260 HPLC coupled with Sciex 5600 QTOF: untargeted metabolomics
Shimadzu LC-30AD UHPLC with Sciex 5500 QTRAP: nucleotides and CoA derivatives analysis
Compound Discoverer Software
Thermo Ultimate 3000 nanoLC coupled with Orbitrap Fusion Lumos (with EasyIC and ETD): discovery preoteomics & quantitative proteomics
Agilent 1260 HPLC coupled with Sciex 5600 QTOF: discovery proteomics and molecular weight analysis of recombinant protein
Software:
Proteome Discoverer offers multiple integrated tools for statistical and biological interpretation to quickly turn data into insights.
Maxquant is a quantitative proteomics software package designed for analyzing large mass-spectrometric data sets.
Skyline provides state of the art modeling, analytics, and visualization software to produce highly accurate and visually stunning 2D and 3D products.
Access Information
We operate under a range of modes for internal, academic collaborations and external scientists. For academic collaborations, we have the capacity to conduct experiments for the collaborator, or provide the collaborator in-house access to conduct key experiments within the facility.
External researchers can book and pay for the use of the facility equipment by first contacting the facility manager.
External researchers can book and pay for the use of the facility equipment by first contacting the facility manager.
For commercial partners, SVI laboratories will perform experiments under subcontract. Our company, Effica Biolabs, can perform pre-clinical studies on immune system and animal models on a contract.
Contacts
Anthony DiCarluccio
Cytometry Manager (including 10x)
adicarluccio@svi.edu.au
Dingyi Yu
Mass Spec Facility Manager
dyu@svi.edu.au
Team Members
Images
HPLC-SCIEX 5500 QTRAP for metabolomics
HPLC-SCIEX 5600 QTOF for intact protein analysis (proteomics, metabolomics)
NanoLC ThermoFusionLumos Orbitrap for proteomics