Doherty Applied Microbial Genomics

The University of Melbourne
Functional Genomics • Transcriptomics • Microbiomics • Genomics

Overview Capabilities Equipment Access Information Contacts Team Members Images

Overview

Within the framework of collaborative partnerships, Doherty Applied Microbial Genomics (DAMG) offers sequencing capacity, established expertise in bioinformatics and critical training to undertake microbial genomics research, which will help laboratories across Australia to breach barriers that are restricting access to this new cutting edge technology. The initiative is funded by the Department of Microbiology and Immunology at The University of Melbourne, and has a close alignment with the Microbiological Diagnostic Unit Public Health Laboratory (MDU PHL).
We have established expertise in bioinformatics and capacity to perform microbial genomics research make us uniquely placed to perform and facilitate research in this rapidly evolving area of clinical and public health microbiology.
Primary research themes include:

Antimicrobial resistance,
Infectious disease surveillance and one health, and
Metagenomics and Software development.

Method specialties include:

Amplicon 16S microbiota profiling,
Shotgun metagenome sequencing, and
Whole Genome Sequencing (WGS) on purified isolates.

Capabilities

Genomics

Bacterial Whole Genome Sequencing (WGS) Viral WGS Metagenomics Targeted WGS

Microbiomics / Pathogen Genomics

High throughput bacterial DNA/RNA extraction

Amplicon sequencing

16S rRNA V4 16S rRNA full gene Custom amplicons

Sequencing of pre-made libraries

Illumina Oxford Nanopore Technology Custom libraries

Long-read sequencing

Oxford Nanopore Tecnnologies PacBio

Transcriptomics

Bacterial Metatranscriptomics

Bioinformatics and Data Analysis

Clinical, Public health and Research Microbiology focused

Equipment

Short-read sequencing

iSeq 100 Sequencing System MiSeq System NextSeq500/500 NextSeq 2000

Long-read sequencing

Oxford Nanopore Technologies GridlON for on-demand sequencing with integrated real-time data processing SMRT Cell Sequencing (Sequel I)

Automated Liquid Handling

Perkin Elmer Zephyr for NGS applications

Automated DNA/RNA preparation

QIAsymphony

Plate Reader

FLUOstar® Omega - multi-mode microplate reader with six detection modes, utilizing an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes.

Access Information

Research collaborations between your organisation and DAMG include project and experimental planning, all laboratory work and bioinformatics analysis and manuscript preparation.

For further information contact us at DAMG-general@unimelb.edu.au or submit a completed project proposal form to begin discussions.

Website: www.doherty.edu.au/genomics

Contacts

General contact
DAMG-general@unimelb.edu.au

Team Members