Peter Mac Molecular Genomics Core

Overview

The Molecular Genomics Core at Peter Mac processes samples, implements and develops new genomics protocols for cancer research and translational applications. We work predominantly with Next Generation Sequencing (NGS, Illumina compatible) but also with the nCounter technology (Nanostring) for gene expression of FFPE-derived samples. We operate a Chromium iX for single-cell sequencing and the Visium (10xGenomics) and GeoMx DSP (Nanostring) for spatial transcriptomics. We specialize in custom protocols and challenging cancer samples such as low input and FFPE-derived. 

Capabilities

Genomics

Whole-genome sequencing (WGS) Whole exome sequencing (WES) DNA targeted capture (off-the-shelf and custom panels) Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-Seq) Chromatin immunoprecipitation followed by sequencing (ChIP-seq) Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) Methylation CRISPR-Seq Support in custom applications

Sequencing of pre-made libraries

NextSeq 500 only

Transcriptomics

3'RNA-seq RNA-Seq (polyA and ribo-depletion) RNAseq and whole exome sequencing (WES) SLAM-Seq PRO-Seq High-throughput low-cost RNA-Seq (MAC-Seq) FFPE-derived RNA Support in custom applications

Equipment

Single-Cell Technologies

Chromium iX (10xGenomics)

Short-read sequencing

Illumina NextSeq Illumina NovaSeq

Spatial Omics Technologies

GeoMx DSP (Nanostring) Visium (10X Genomics)

Automated Liquid Handling

Bravo BenchCel Workstation EpMotion

Other instrumentation

nCounter Nanostring for gene expression compatible with FFPE-derived RNA

Access Information

For further information visit www.petermac.org/research/core-facilities-and-services/molecular-genomics

Contacts

General contact
ResearchMolecularGenomics@petermac.org

Tim Semple
Research Officer
tim.semple@petermac.org

Team Members