Melbourne Mass Spectrometry and Proteomics Facility

The University of Melbourne
Metabolomics • Lipidomics • Proteomics • Single Cell

Overview Capabilities Equipment Access Information Contacts Images

Overview

The Melbourne Mass Spectrometry and Proteomics Facility (MMSPF) located at the Bio21 Institute is one of the largest mass spectrometry facilities in Australia. The MMSPF is an open access facility that provides academic and industry researchers access to specialist mass spectrometry and proteomic equipment and expertise.

The Facility has 15 mass spectrometer based systems and five full time Ph.D. staff dedicated to providing all experimental design, training, machine time, and informatics support required to execute any proteomics or lipidomics research project. The facility provides all users direct access to instrumentation and trains them to perform their own proteomics or lipidomics experiments.

Techniques such as protein identification (e.g. Gel spots, or immuno-precipitated protein), full scale proteomics (TMT, SILAC, DIA, Label free, etc), phosphoproteomics , N-Terminomics (in-vivo protease activity), protein structural characterisation (PTM's, disulphide arrangement, HDX, Crosslinking).

The facility has the following instrumentation:
• 2 x Thermo OrbiTrap ASTRAL Zoom

• 2 x Thermo OrbiTrap ASTRAL

• Thermo OrbiTrap Eclipse

• Thermo OrbiTrap Ascend ETD

• Thermo OrbiTrap Exploris 480

• Thermo OrbiTrap Exactive plus

• Agilent 6545 QTOF

• Agilent Revident QTOF

• Waters XS IMS QTOF

• Waters HDX OrbiTrap Exploris 480 system

• Thermo Altis Plus QQQ

• Shimadzu 8030 MALDI-TOF

• Shimadzu 8050 QQQ

Capabilities

Equipment

Metabolomics Equipment

Shimadzu 8050 QQQ - used for targetted analysis of pharmaceuticals or small molecules (also used for proteomics)

Proteomics Equipment

All of the following for different proteomic experiments: Thermo OrbiTrap Fusion Lumos ETD, 2 x Thermo Q Exactive Plus mass spectrometer 2 x Thermo OrbiTrap Eclipse mass spectrometer Thermo OrbiTrap Exploris 480 Waters XS IMS QTOF mass spectrometer Waters HDX Synapt system Bruker TIMS TOF Pro Agilent 6530 QTOF Shimadzu 9030 QT

Mass spectrometry

Agilent 8900 QQQ Inductively Coupled Plasma Mass Spectrometry -used for general cellular elemental (metals) analysis and metalloproteomics

Lipidomics

Thermo OrbiTrap Fusion Lumos UVPD - Used for both untargetted and targetted analysis of Lipid samples

Access Information

For further information visit https://research.unimelb.edu.au/facilities/infrastructure/mass-spectrometry-proteomics-facility

Contacts