St Vincent's Institute Technologies Platform

Overview

SVI focus on a wide range of disease-oriented research: 
  • Cancer
  • Type 1 diabetes
  • Type 2 diabetes and obesity
  • Heart disease
  • Bone diseases, including osteoporosis
  • Alzheimer's disease

Our in-house tools include: 
  • Flow Cytometry (analysers + sorters)
  • 3 × LC-MS for intact protein, proteomics & metabolomics analysis 
  • 10x Genomics Chromium controller

Capabilities

Proteomics

Protein Identification Qualitative & quantitative proteome analysis Molecular weight analysis of purified protein samples

Metabolomics

Untargeted metabolomics Nucleotides & Co-enzyme A (CoA) derivatives analysis

Transcriptomics

Construction of long-read (PacBio) Short-read (Illumina) and single cell RNA sequencing libraries by several labs, especially the Genomics & Immunology laboratory

Single-cell omics

10x Genomics Chromium controller

Compounds

Quanlitative analysis by high-resolution mass spectrometry Drug purity check C18 fractionation on semi-preparative HPLC

Bioinformatics and Data Analysis

Bioinformatics capabilities located in within the following research groups: 1. Bioinformatics & Cellular Genomics, 2. Genomics & Immunology, and 3. Cancer & RNA Biology.

Equipment

Single-Cell Technologies

10x Genomics Chromium controller

Real-time PCR / qPCR / Digital droplet PCR (ddPCR)

Numerous qPCR instruments, including ABI Quantstudio, Lightcycler, etc

Metabolomics Equipment

Thermo Ultimate 3000 LC coupled with Orbitrap Fusion Lumos Agilent 1260 HPLC coupled with Sciex 5600 QTOF: untargeted metabolomics Shimadzu LC-30AD UHPLC with Sciex 5500 QTRAP: nucleotides and CoA derivatives analysis Compound Discoverer Software

Proteomics Equipment

Thermo Ultimate 3000 nanoLC coupled with Orbitrap Fusion Lumos (with EasyIC and ETD): discovery preoteomics & quantitative proteomics Agilent 1260 HPLC coupled with Sciex 5600 QTOF: discovery proteomics and molecular weight analysis of recombinant protein Software: Proteome Discoverer offers multiple integrated tools for statistical and biological interpretation to quickly turn data into insights. Maxquant is a quantitative proteomics software package designed for analyzing large mass-spectrometric data sets. Skyline provides state of the art modeling, analytics, and visualization software to produce highly accurate and visually stunning 2D and 3D products.

Access Information

We operate under a range of modes for internal, academic collaborations and external scientists. For academic collaborations, we have the capacity to conduct experiments for the collaborator, or provide the collaborator in-house access to conduct key experiments within the facility.

External researchers can book and pay for the use of the facility equipment by first contacting the facility manager.
 
For commercial partners, SVI laboratories will perform experiments under subcontract. Our company, Effica Biolabs, can perform pre-clinical studies on immune system and animal models on a contract.


Contacts

Dingyi Yu
Mass Spec Facility Manager
dyu@svi.edu.au

Anthony DiCarluccio
Cytometry Manager (including 10x)
adicarluccio@svi.edu.au

Team Members


 

Images