WEHI Proteomics Facility

Overview

The Proteomics Facility at WEHI enables researchers and clinicians to utilise the advanced proteomic technologies available at WEHI. We employ five full-time, post-doctoral researchers and have seven mass spectrometers for analysis of proteomics samples. We charge users the hours spent by facility staff on preparation of samples, instrument time (data acquisition on the mass spectrometer) and data analysis.

Capabilities

Proteomics

Global proteome profiling Immunoprecipitation analysis Intact mass spectrometry TurboID BioID Cell surface labeling Phosphoproteomic analysis PTM analysis Cross-linking mass spectrometry (XL-MS) DIA proteomics Clinical cohort analysis Targeted mass spectrometry (PRM)

Single-cell omics

mTRAQ-based proteomics Tandem Mass Tag (TMT) -based proteomics

Equipment

Single-Cell Technologies

timsTOF Pro (Bruker) Orbitrap Eclipse Tribrid (Thermo)

Automated Liquid Handling

LEAP HDX Automated Robot (Trajan)

Plate Reader

Lunatic - UV/Vis Absorbance Spectrometer

Proteomics Equipment

timsTOF Pro (Bruker) Orbitrap Eclipse Tribrid (Thermo) maXis II QTOF (Bruker) Q-Exactive Classic (Thermo)

Access Information

Contacts

Laura Dagley
Facilty Manager
dagley.l@wehi.edu.au

Samantha Emery
Interim Facility Manager
emery.s@wehi.edu.au

A/Prof Andrew Webb
Centre Head and Proteomics Lab Head
webb@wehi.edu.au

Team Members

  • Laura Dagley, facility manager | point of contact for facility access, protoemic experimental design and planning and handover of samples | dagley.l@wehi.edu.au
  • Samantha Emery | general proteomics - global proteome profiling, clinical proteomcs, plasma proteomics, label-free quantitation | emery.s@wehi.edu.au 
  • Toby Dite, post-translation modification (PTM) specialist | PMT identification and localisation, intact MS, proximity biotin labelling, surface labelling |  dite.t@wehi.edu.au
  • Jumana Yousef,  proteomics biostatistician | experimental design, data processing and quality control, statistical analyses of proteomic data