ONJCRI Cancer Single Cell Genomics Laboratory

Overview

The Cancer Single Cell Genomics Laboratory (CSCG) specialises in using innovative single cell techniques to deconstruct tissue samples and reveal the genetic architecture of individual cells and offers training, advice on experimental design and collaboration options. 

The ONJ Cancer Centre is also home to the ACRF Centre for Imaging the Tumour Environment (CITE) which can provide researchers with the information required for the development of effective anti-cancer therapies. CITE offers one-on-one customised training, advice on experimental design and collaboration options. Researchers are supported at all stages of their project from design through to image analysis and reporting.

Capabilities

Genomics

Human whole-genome sequencing (WGS)

Single Cell Omics

scTranscriptomics, scATACseq, scTCRseq (mouse and human TCRα/β and 𝛾𝛿 TCR repertoires)

Spatial Omics

Fresh frozen optimal cutting temperature (OCT) Formalin-fixed, paraffin-embedded (FFPE) tissue preservation

Equipment

Live-Cell Imaging

Fully automated Olympus IX81 microscope with environmental chamber

Other Instrumentation

Tapestation2000 for RNA/DNA estimation and analysis

Imaging

•Confocal microscopy: Zeiss 980 with Airyscan and incubator for live cell imaging - inverted, Zeiss 980 with Airyscan and incubator for live cell imaging - upright •Multiphoton microscope: Zeiss 980 with dual line Insight X3 pulsed laser •Slide scanner-automated: Leica Aperio AT2 with 400 slide capacity •Microscope with multispectral camera: Vectra 3 with 200 slide loader •Automated immunostainer: Leica BOND RX •Image Analysis: HALO/AI (IndicaLabs), Metamorph (Molecular Devices), Inform (Akoya), Phenochart (Akoya), FIJI, Aperio ImageScope (Leica)

Single-Cell Technologies

10x Genomics' Chromium

Spatial Omics Technologies

10x Genomics' Chromium

Real-time PCR / qPCR / Digital droplet PCR (ddPCR)

BioRad realtime qpCR and digital droplet PCR

Automated Liquid Handling

Perkin Elmer for 96 well plates

Access Information

The Cancer Single Cell Genomics Laboratory (CSCG) is open to internal and external collaborations on omics projects. The ONJCRI and visiting scientists are offered training and encouraged to perform research.

For further information visit www.onjcri.org.au/our-research/cancer-single-cell-genomics-laboratory/

Contacts

Dr Bhupinder Pal
Head, CSCG
bhupinder.pal@onjcri.org.au

Sarah Ellis
Head, CITE
Sarah.Ellis@onjcri.org.au

Team Members

Images